Ligation mediated fluorescent labeling of DNA sequencing primers
نویسندگان
چکیده
منابع مشابه
Substrate properties of fluorescent ribonucleotides in the terminal transferase-catalyzed labeling of DNA sequencing primers.
Terminal deoxynucleotidyltransferase (terminal transferase, E.C. 2.7.7.31) has been used to add a single fluorescent ribonucleotide to the 3' terminus of DNA sequencing primers, thereby creating primers suitable for automated DNA sequence analysis. The previously introduced procedure using fluorescein-UTP for the postsynthetic labeling of primers can, under appropriate reaction conditions, now ...
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DNA primer sets, labeled with two fluorescent dyes to exploit fluorescence energy transfer (ET), can be efficiently excited with a single laser line and emit strong fluorescence at distinctive wavelengths. Such ET primers are superior to single fluorophore-labeled primers for DNA sequencing and other multiple color-based analyses [J. Ju, C. Ruan, C. W. Fuller, A. N. Glazer and R. A. Mathies (19...
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1.Atcheson, C.L., B. DiDomenico, S. Frackman, R.E. Esposito and R.T. Elder. 1987. Isolation, DNA sequence, and regulation of a meiosis-specific eukaryotic recombination gene. Proc. Natl. Acad. Sci. USA 84:80358039. 2.Cha, J., W. Bishai and S. Chandrasegaran. 1993. New vectors for direct cloning of PCR products [published erratum appears in Gene 1994; 141:149]. Gene 136:369-370. 3.Clark, J.M. 19...
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DNA sequence information is required for many routine applications of current molecular biology. Although sequence data may be generated quickly and efficiently using dedicated automated DNA sequencers, many small laboratories do not have the sequencing demands or finances to justify their purchase. For instance, a primary objective of the U.S. Fish and Wildlife Service’s Fish Genetics Laborato...
متن کاملSequencing by Ligation with Double-Labeled Fluorescent Probes
We have synthesized a set of double-labeled fluorescent probes and evaluated the performance of these fluorescent probes in sequencing by ligation (SBL). The results indicated that the ligation efficiency and specificity of double-labeled fluorescent probes was as higher as that of singe-labeled fluorescent probes. Moreover, double-labeled f luorescent p robes y ielded a r emarkable i ncrease i...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1997
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/25.4.922